The INV-3i FL Inverted Fluorescence Microscope is designed for live-cell and fluorescence imaging applications in life-science research. It combines an infinite optical system, trinocular viewing head, long-working-distance objectives, 100W mercury fluorescence illumination, specialized excitation filters, phase-contrast capability and precision coaxial focusing.

Product Overview

The INV-3i FL Inverted Fluorescence Microscope is an inverted microscopy platform designed for examining specimens positioned on top of the stage, making it particularly suitable for cultured cells in flasks, Petri dishes and other culture vessels.

The microscope uses an infinite optical system and a 30° inclined trinocular head with an interpupillary distance of 48–75 mm. Its quintuple nosepiece accommodates the specified long-working-distance and phase objectives.

The optical configuration includes an EW10X/22 extra-wide-field eyepiece, LWD Infinite Plan 4X and 40X objectives, and Infinite Plan Phase 10X and 20X objectives. The specified optical combination provides multiple magnification options for routine observation, phase-contrast work and fluorescence imaging.

For fluorescence applications, the system incorporates a 100W mercury lamp, blue and green excitation filters, and a UV-resistant protection barrier. The blue excitation filter is specified at 460–490 nm, while the green excitation filter is specified at 480–550 nm.

A fixed phase annulus plate covering 10X–20X and 40X phase applications, together with an ELWD condenser, supports observation of living cells and other specimens requiring enhanced contrast.

Key Features and Benefits

• Infinite optical system
• 30° inclined trinocular head
• 48–75 mm adjustable interpupillary distance
• Quintuple nosepiece
• 100W mercury fluorescence illumination
• UV-resistant protection barrier
• Blue excitation filter: 460–490 nm
• Green excitation filter: 480–550 nm
• EW10X/22 extra-wide-field eyepiece
• LWD Infinite Plan 4X and 40X objectives
• Infinite Plan Phase 10X and 20X objectives
• ELWD condenser with NA 0.3
• 72 mm condenser working distance
• Fixed phase annulus plate
• Mechanical X-Y stage with 120 × 78 mm movement
• Coaxial coarse and fine focusing
• 0.002 mm fine-focus division
• 6V/30W halogen transmitted illumination
• Blue, green and ground-glass filters for transmitted illumination

4. Technical Specifications

5. Applications

• Live-cell imaging
• Cell biology research
• Cancer research
• Neuroscience research
• Developmental biology
• Pharmaceutical drug discovery
• Cell-based assays
• Microbiology research
• Virology research
• Cell culture observation
• Biomanufacturing quality control
• Biologics and cell-therapy research
• Fluorescence microscopy
• Phase-contrast microscopy

6. Suitable For

• Life-science research laboratories
• Cell biology laboratories
• Cancer research centres
• Pharmaceutical R&D laboratories
• Biotechnology laboratories
• Neuroscience laboratories
• Developmental biology research
• Cell culture facilities
• Drug discovery laboratories
• Microbiology and virology research laboratories
• Biomanufacturing facilities
• Academic research institutions

7. Product Benefits

• Inverted configuration: Allows observation of specimens in culture vessels positioned above the objectives.
• Fluorescence capability: 100W mercury illumination provides the dedicated fluorescence excitation source specified for the system.
• Multiple excitation bands: Blue and green excitation filters provide two specified fluorescence excitation ranges.
• Live-cell suitability: The inverted design and long-working-distance optics are suited to observation of cultured cells.
• Phase-contrast capability: Fixed phase annuli and phase objectives support contrast-enhanced observation of suitable specimens.
• Long working distance: LWD objectives and ELWD condenser provide working distances suited to culture vessels.
• Trinocular imaging: The trinocular head provides a dedicated configuration for visual observation and camera attachment.
• Precision focusing: Coaxial coarse and fine controls provide controlled focusing, with 0.002 mm fine division.
• Flexible specimen movement: The mechanical X-Y stage enables controlled movement across the specimen.
• UV protection: A dedicated protection barrier is incorporated to resist ultraviolet light from the fluorescence illumination system.