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Product Description

The Cresta ZS-50i FL Inverted Fluorescence Microscope combines an inverted research microscope platform with 100W DC mercury fluorescence illumination, dual fluorescence filter sets and a trinocular imaging head. It is designed for fluorescence, brightfield and phase-contrast microscopy, including live-cell and cell-culture applications.

Product Overview

The Cresta ZS-50i FL is an inverted fluorescence microscope built on the Cresta ZS-50i platform. It combines a rigid microscope structure, Siedentopf trinocular head, precision coaxial focusing, mechanical X-Y stage and built-in transmitted LED illumination with a dedicated reflected-light fluorescence module.

The fluorescence module uses a 100W DC mercury lamp house and includes blue and green fluorescence filter sets. The blue filter set has 450–490 nm excitation, a 510 nm dichroic mirror and 520 nm emission barrier, while the green filter set has 480–550 nm excitation, a 570 nm dichroic mirror and 590 nm emission barrier.

The trinocular head splits the optical path between the eyepieces and camera port and is specified as compatible with a wide range of C-mount cameras. A UV protection barrier is included for fluorescence operation.

The inverted configuration is particularly suited to specimens in culture vessels and supports applications such as live-cell fluorescence imaging, immunofluorescence, FISH and fluorescence-based cell assays.

Key Features and Benefits

  • 100W DC mercury lamp for fluorescence illumination
  • Blue and green fluorescence filter sets
  • Blue excitation: 450–490 nm
  • Green excitation: 480–550 nm
  • FITC / Alexa Fluor 488 compatible blue filter set
  • TRITC / Cy3 / Texas Red compatible green filter set
  • 510 nm and 570 nm dichroic mirrors
  • 520 nm and 590 nm emission barriers
  • UV protection barrier
  • Inverted microscope configuration
  • Siedentopf trinocular head
  • 30° inclined, 360° rotatable head
  • Reversed inward quadruple ball-bearing nosepiece
  • Coaxial coarse and fine focusing
  • 0.002 mm fine-focus divisions
  • Ball-bearing mechanical X-Y stage
  • Movable Abbe condenser, NA 1.25
  • Built-in transmitted LED illumination
  • C-mount camera compatibility
  • Brightfield and phase-contrast capability retained from the base platform

4. Technical Specifications

Specification Details
Product Model Cresta ZS-50i FL
Microscope Type Inverted Fluorescence Microscope
Base Microscope Cresta ZS-50i
Optical Configuration Inverted
Viewing Head Siedentopf Trinocular
Head Inclination 30°
Head Rotation 360°
Nosepiece Reversed inward quadruple ball-bearing
Nosepiece Positioning Positive click stops
Focusing Coaxial coarse & fine
Fine Focus Graduation 0.002 mm
Stage Ball-bearing mechanical stage
Condenser Movable Abbe condenser
Condenser NA 1.25
Transmitted Illumination Built-in LED
LED Control Intensity control
Fluorescence Light Source Mercury lamp house
Mercury Lamp 100W DC
Fluorescence Mode Reflected-light / epi-fluorescence
Blue Excitation 450–490 nm
Blue Dichroic Mirror 510 nm
Blue Emission Barrier 520 nm
Green Excitation 480–550 nm
Green Dichroic Mirror 570 nm
Green Emission Barrier 590 nm
Blue Filter Applications FITC, Alexa Fluor 488
Green Filter Applications TRITC, Cy3, Texas Red
Filter Configuration Slider or revolving turret
UV Protection UV protection barrier included
Camera Interface C-mount compatible
Imaging Trinocular camera and eyepiece light split
Structure Single-mould rigid structure
Additional Capability Brightfield and phase contrast

5. Applications

  • Live-cell fluorescence imaging
  • Immunofluorescence
  • Immunocytochemistry
  • Fluorescence in situ hybridization (FISH)
  • Cell viability assays
  • Apoptosis studies
  • Cell proliferation and division studies
  • Fluorescent protein imaging
  • GFP-based research
  • Calcium imaging
  • Intracellular pH studies
  • Neuroscience research
  • Developmental biology
  • Pharmaceutical research
  • Biotechnology research
  • Clinical fluorescence microscopy

6. Suitable For

  • Cell biology laboratories
  • Biotechnology research laboratories
  • Pharmaceutical R&D
  • Academic research institutions
  • Neuroscience laboratories
  • Developmental biology laboratories
  • Cell-culture facilities
  • Immunology laboratories
  • Molecular biology laboratories
  • Clinical research laboratories
  • Fluorescence imaging facilities

7. Product Benefits

  • Inverted fluorescence platform: Suitable for observing specimens in culture vessels and other inverted-microscopy workflows.
  • High-intensity fluorescence source: The 100W DC mercury lamp provides the specified fluorescence illumination.
  • Dual filter capability: Blue and green filter sets support commonly used fluorophore groups.
  • Camera-ready design: The trinocular head provides a dedicated imaging path and C-mount compatibility.
  • Multi-mode microscopy: The base platform retains brightfield and phase-contrast capabilities.
  • Precision focusing: Coaxial coarse/fine focusing with 0.002 mm divisions provides controlled focusing.
  • Smooth specimen positioning: Ball-bearing mechanical stage supports X-Y movement.
  • Operator protection: A UV protection barrier is included for fluorescence operation.
  • Flexible filter mounting: Filter sets can be housed in a slider or revolving turret.
  • Research versatility: Suitable for fluorescence imaging alongside conventional inverted microscopy applications.

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