Fluorescence Spectrophotometers provide valuable insights into fluorescent compounds’ structural and functional properties and enable researchers to perform sensitive and selective analyses.

Fluorescence Spectrophotometer is a scientific instrument used to measure the fluorescence properties of a substance. It operates based on the principle that certain compounds, when exposed to light of a specific wavelength, absorb that light energy and then emit light of a longer wavelength, a phenomenon known as fluorescence.

• Light Source: Fluorescence spectrophotometers typically use a high-energy light source, such as a xenon lamp or a mercury arc lamp, to provide the excitation light. This light source emits light across a range of wavelengths.
• Monochromators: These optical filters select specific wavelengths of light from the light source. In a fluorescence spectrophotometer, there are typically two monochromators: one for selecting the excitation wavelength and the other for selecting the emission wavelength. This allows researchers to control the specific wavelengths of light used for excitation and detection.
• Sample Compartment: This is where the sample to be analyzed is placed. The sample is typically contained in a quartz cuvette, which is transparent to the wavelengths of light used in fluorescence spectroscopy.
• Detector: After the light source excites the sample, it emits fluorescence at longer wavelengths. The detector in the spectrophotometer measures the intensity of this emitted fluorescence. Photomultiplier tubes (PMTs) are commonly used as detectors due to their high sensitivity to low levels of light.
• Data Processing and Display: The signals from the detector are processed by the instrument’s electronics and then displayed on a screen or recorded for further analysis. This data can provide valuable information about the properties of the sample, such as its concentration or chemical composition.

Technical Specification

Excitation Light Source High Brightness LED
Excitation LED 250nm ~ 600nm(LED)
EX Wavelength Standard set: 365nm,376nm,392nm,405nm
Emission Monochromator C-T configuration diffraction grating monochromator Emission
Wavelength range (EM): 200nm~650nm, Bandwidth: 10nm
(Extend the Monochromator to Em200-900 is Optional)
Emission Wavelength Accuracy: ± 1nm
Emission Wavelength Reproducibility:≤0.5nm
S/N Ratio S/N≥90 (Using 1cm Quartz Sample Cell, Measure the
Signal Noise Ratio of Raman Spectrum of Water )
Detection Limit 1×10-10g / ml Quinine Sulfate Solution
Linearity (γ) ≥0.995
Repetitive Peak Intensity ≤1.5%
Zero Drift ≤0.3(within 10min
Power Type 220V±22V; 110V±22V
Dimensions 442 x 392 x 250 (mm)
Weight Net Weight 10kg
Gross Weight 12k

Frequently Asked Questions (FAQs)

1. What is a Fluorescence Spectrophotometer?

A Fluorescence Spectrophotometer is an analytical instrument used to measure fluorescent light emitted by a sample after excitation with light of a specific wavelength. It is commonly used for sensitive chemical and biological analysis. 

2. How does a fluorescence spectrophotometer work?

The instrument excites the sample using a selected wavelength of light. The sample emits fluorescence at a longer wavelength, which is detected and analyzed to determine concentration and molecular properties. 

3. Which industries use fluorescence spectrophotometers?

These instruments are widely used in:

• Pharmaceutical laboratories

• Biotechnology research centers

• Environmental testing labs

• Food and beverage industries

• Academic and research institutions

• Clinical and diagnostic laboratories

4. What are the major applications of fluorescence spectroscopy?

Applications include DNA/RNA quantification, protein analysis, pharmaceutical testing, fluorescence assays, pollutant detection, enzyme kinetics, and biomolecular research.

5. What type of detector is used in fluorescence spectrophotometers?

Most fluorescence spectrophotometers use highly sensitive Photomultiplier Tube (PMT) detectors for low-level light detection. 

6. What light source is commonly used?

The instrument commonly uses a Xenon lamp, LED source, or Mercury arc lamp for excitation. 

7. Can the instrument perform quantitative analysis?

Yes, fluorescence spectrophotometers are highly effective for quantitative analysis by measuring fluorescence intensity relative to calibration standards. 

8. Is the fluorescence spectrophotometer suitable for DNA and protein analysis?

Yes, the instrument is widely used for DNA, RNA, protein quantification, and molecular biology applications due to its high sensitivity. 

9. What are the advantages of fluorescence spectroscopy?

Major advantages include:

• High sensitivity

• Trace-level detection

• Excellent selectivity

• Rapid analysis

• Non-destructive testing 

10. Why should I buy a Fluorescence Spectrophotometer from Labmart India?

Labmart India offers premium laboratory instruments, competitive pricing, technical support, reliable after-sales service, fast delivery across India, and trusted solutions for research and industrial laboratories. 

Application Areas

Pharmaceutical Industry

Used for drug discovery, pharmaceutical formulation analysis, fluorescence assays, and quality control testing.

Biotechnology & Life Sciences

Ideal for DNA/RNA quantification, protein studies, enzyme kinetics, cell analysis, and biomolecular interaction studies.

Environmental Testing Laboratories

Applicable for detection of pollutants, toxins, organic compounds, and environmental contaminants in water, soil, and air samples.

Clinical & Diagnostic Laboratories

Supports biomarker detection, medical diagnostics, disease research, and fluorescence-based clinical testing.

Food & Beverage Industry

Used for contaminant detection, nutrient analysis, food quality testing, and additive analysis.

Academic & Research Institutions

Widely used in universities, colleges, and scientific research laboratories for spectroscopy and fluorescence studies.

Chemical & Material Science Laboratories

Suitable for molecular characterization, fluorescence emission studies, and material property analysis.

Nanotechnology & Advanced Research

Used in nanoparticle analysis, quantum dot studies, fluorescent polymer research, and advanced material characterization.