A Nano Spectrophotometer is a specialized instrument used to measure the absorption and concentration of nucleic acids (DNA, RNA) and proteins in very small volumes, typically in the nanoliter range. It is commonly employed in molecular biology, biotechnology, and life science research for various applications, including DNA/RNA quantification, protein analysis, and purity assessment of biomolecules.

Nano Spectrophotometer Model No: LI-5210 is a compact and precise instrument designed for quantitative analysis of DNA, RNA, and proteins in small sample volumes. Here’s a detailed description of its features and specifications:

Key Features:

1. Compact Design: The LI-5210 is designed to occupy minimal bench space while providing high functionality, making it ideal for small labs and crowded workspaces.
2. Small Sample Volume: Capable of analyzing samples as small as 0.5-2 µL, which is particularly useful for precious or limited samples.
3. High Sensitivity and Accuracy: The spectrophotometer offers highly accurate and reproducible measurements, essential for reliable quantitative analysis.
4. Wide Wavelength Range: It typically covers a broad wavelength range (e.g., 190-850 nm), allowing for diverse applications in molecular biology and biochemistry.
5. User-Friendly Interface: Often equipped with a touch screen or simple user interface for easy operation and data management.
6. Rapid Measurements: Provides quick readout times, facilitating high-throughput sample analysis.
7. Calibration and Maintenance: Usually includes auto-calibration features and minimal maintenance requirements, enhancing user convenience.

Applications:

• Nucleic Acid Quantification: Accurate measurement of DNA, RNA concentrations and purity.
• Protein Analysis: Quantification and quality assessment of protein samples.
• Micro-volume Analysis: Ideal for scenarios where sample volume is limited.
• Quality Control: Suitable for use in research, clinical, and industrial laboratories to ensure sample integrity and consistency.

Accessories:

• Sample Pedestal: For easy loading and cleaning of micro-volume samples.
• Cuvettes: Optional cuvette holder for larger sample volumes.
• Software Suite: Data analysis and management software tailored for molecular biology applications.

Operation:

1. Sample Loading: Place a small volume of the sample on the pedestal or in a cuvette.
2. Measurement: Initiate the measurement via the touch screen or connected computer.
3. Data Analysis: The instrument provides real-time data and can store or export results for further analysis.

General Specification

-Light Source Xenon Flash Lamp
-Detector CCD (2,048 Pixels), (reference beam available)
-Wavelength Accuracy ±1 nm
-Wavelength Range 190 – 1,100 nm
-Spectral Resolution 0.3 nm
-Dimension 220 x 280 x 220 mm, 8.6 x 11.0 x 8.6 in
-Weight 5 kg
-Operator Voltage 100 – 240 V, 50 – 60 Hz
-Power Consumption 80 – 100 W
-1 Warranty Year
-LCD Touch Panel 7.0 in

Nano Volume Specification

-Absorbance Precision 1% at 100 ng/µl
-Absorbance Range 0 – 300 Abs. (10 nm equivalent)
-Detection Limit 2 ng/µl (dsDNA)
-Maximum Concentration 15,000 ng/µl (dsDNA)
-Measurement T 5 sec ime
-1 µl Minimum Sample Size
-Path Length 0.01 – 1.2 mm (Auto-ranging)

Cuvette Specification

-Absorbance Range    0.002 – 2.0 Abs.
-Measurement time          3 sec
-Beam height                 8.5mm